Composite

Part:BBa_K2959000:Design

Designed by: Alejandro Aguirre Hernndez   Group: iGEM19_Tec-Chihuahua   (2019-08-26)


Expressible LL 37 - Cathelicidin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LL 37 has been found to have antimicrobial activity against Gram-negative bacteria including E. coli. Due to the toxicity of the peptide against the chosen chassis, an inducilbe promoter was chosen to control the expression of the part. iGEM12_Trieste optimized the sequence of LL 37 for E. coli expression.


Source

This composite part was constructed using previously existing parts in the Registry: Promoter T7 + RBS (BBa_K525998), LL 37 - Cathelicidin (BBa_K875009), and double terminator (BBa_B0015).

References